The generation of immunotoxins using chimeric anti-CD22 antibodies containing mutations which alter their serum half-life

Laurentiu M Pop; Xiaoyun Liu; Victor Ghetie; Ellen S Vitetta

doi:10.1016/j.intimp.2005.03.013

The generation of immunotoxins using chimeric anti-CD22 antibodies containing mutations which alter their serum half-life

Int Immunopharmacol. 2005 Jul;5(7-8):1279-90. doi: 10.1016/j.intimp.2005.03.013. Epub 2005 Apr 26.

Authors

Laurentiu M Pop¹, Xiaoyun Liu, Victor Ghetie, Ellen S Vitetta

Affiliation

¹ The Cancer Immunobiology Center, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., NB99.210 Dallas, TX 75390-8576, USA.

PMID: 15914332
DOI: 10.1016/j.intimp.2005.03.013

Abstract

Murine and chimeric RFB4 (anti-human CD22) monoclonal antibodies (MAbs) with mutations in their Fc portions were conjugated to recombinant ricin toxin A chain to generate immunotoxins. The resulting immunotoxins (ITs) constructed with chimeric RFB4 MAbs were designed to have longer or shorter half-lives but similar binding and cytotoxic properties. These ITs can now be evaluated in vivo for improved therapeutic indices. The characteristics of these ITs are the subject of this report.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / pharmacokinetics*
Antigens, CD / immunology*
Antigens, Differentiation, B-Lymphocyte / immunology*
Cell Adhesion Molecules / immunology*
Cell Line
Female
Half-Life
Humans
Immunotoxins / pharmacokinetics*
Immunotoxins / pharmacology
Lectins / immunology*
Mice
Mutation
Ricin / pharmacokinetics*
Sialic Acid Binding Ig-like Lectin 2
U937 Cells

Substances

Antibodies, Monoclonal
Antigens, CD
Antigens, Differentiation, B-Lymphocyte
CD22 protein, human
Cd22 protein, mouse
Cell Adhesion Molecules
Immunotoxins
Lectins
Sialic Acid Binding Ig-like Lectin 2
Ricin