Total and reactive lysine contents in selected cereal-based food products

J Agric Food Chem. 2005 Jun 1;53(11):4454-8. doi: 10.1021/jf050071n.

Abstract

The aim of the study was to determine and compare reactive and total lysine contents in a range of breakfast cereal products. Crude fiber, fat, ash, and crude protein contents of 20 breakfast cereal products ranged from 4 to 38, 14 to 144, 7 to 32, and 52 to 253 g/kg, respectively. The concentrations of glutamic acid (18.7-32.1 g/100 g protein) and proline (4.7-10.8 g/100 g protein) were high while those of the amino acids methionine (1.2-2.0 g/100 g protein) and histidine (1.2-3.3 g/100 g protein) were relatively low. There was a strong relationship between reactive lysine determined using the guanidination and fluorodinitrobenzene methods (R = 0.99). The total lysine content, determined after conventional acid hydrolysis, ranged from 0.8 to 3.7 g/100 g protein, while the reactive lysine content (guanidination) ranged from 0.4 to 2.8 g/100 g protein. Reactive lysine was 20-54% lower than total lysine in the cereal products. The large differences between total and reactive lysine suggest a considerable loss of lysine in the breakfast cereals tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Dietary Fats / analysis
  • Dietary Fiber / analysis
  • Dietary Proteins / analysis
  • Dinitrofluorobenzene / chemistry
  • Edible Grain / chemistry*
  • Guanidine / chemistry
  • Lysine / analysis*
  • Lysine / chemistry

Substances

  • Amino Acids
  • Dietary Fats
  • Dietary Fiber
  • Dietary Proteins
  • Dinitrofluorobenzene
  • Guanidine
  • Lysine