Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis

Reiko Nagata; Yoshihiro Muneta; Kazuhiro Yoshihara; Yuichi Yokomizo; Yasuyuki Mori

doi:10.1128/IAI.73.6.3778-3782.2005

Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis

Infect Immun. 2005 Jun;73(6):3778-82. doi: 10.1128/IAI.73.6.3778-3782.2005.

Authors

Reiko Nagata¹, Yoshihiro Muneta, Kazuhiro Yoshihara, Yuichi Yokomizo, Yasuyuki Mori

Affiliation

¹ Immune System Section, Department of Immunology, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan 305-0856. kikuma@affrc.go.jp

Abstract

Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Motifs
Animals
Antigens, Bacterial / chemistry
Antigens, Bacterial / genetics*
Antigens, Bacterial / immunology
Cattle
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Interferon-gamma / biosynthesis*
Mycobacterium avium subsp. paratuberculosis / immunology*
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / immunology

Substances

Antigens, Bacterial
Recombinant Proteins
Interferon-gamma