Abstract
[reaction: see text]. The antitumor antibiotics radicicol and geldanamycin are potent inhibitors of the Hsp90 protein folding machinery. Radester is a hybrid composed of radicicol's resorcinol ring and geldanamycin's quinone through an isopropyl ester. Radester was prepared, and the cytotoxicity of it and the corresponding hydroquinone were determined in MCF-7 breast cancer cells to be 13.9 and 7.1 microM, respectively. Protein degradation assays were performed on Hsp90-dependent client proteins, Her-2 and Raf, to correlate Hsp90 inhibition to cytotoxicity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacology*
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Benzene Derivatives / chemical synthesis
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Benzene Derivatives / chemistry
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Benzene Derivatives / pharmacology*
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Benzoquinones
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Drug Screening Assays, Antitumor
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HSP90 Heat-Shock Proteins / antagonists & inhibitors*
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Humans
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Inhibitory Concentration 50
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Lactams, Macrocyclic
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Oxidation-Reduction
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Phenols / chemistry
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Phenols / pharmacology
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Pyrazoles / chemistry
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Pyrazoles / pharmacology
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Quinones / chemistry
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Quinones / pharmacology
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Structure-Activity Relationship
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Tumor Cells, Cultured
Substances
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Antineoplastic Agents
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Benzene Derivatives
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Benzoquinones
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G3130
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HSP90 Heat-Shock Proteins
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KOSN1559
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Lactams, Macrocyclic
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Phenols
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Pyrazoles
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Quinones
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radester
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geldanamycin