Novel functional biodegradable polymer. III. The construction of poly(gamma-glutamic acid)-sulfonate hydrogel with fibroblast growth factor-2 activity

J Biomed Mater Res A. 2005 Jun 15;73(4):485-91. doi: 10.1002/jbm.a.30305.

Abstract

Poly(gamma-glutamic acid)-sulfonate (gamma-PGA-S) hydrogel, with fibroblast growth factor (FGF)-2 activity was investigated as a novel, next-generation tissue-engineering material. gamma-PGA-net-gamma-PGA-S72 (S72-netgel) was prepared with gamma-PGA-S and gamma-PGA-S72 (72% sulfonated gamma-PGA) to provide the high mobility of gamma-PGA-S72 for FGF-2 activity. Cell adhesion and proliferation activities were evaluated on gamma-PGA and gamma-PGA-S hydrogels along with S72-netgels. Both cell adhesion and proliferation activities of gamma-PGA and gamma-PGA-S hydrogels were low. In contrast, S72-netgels had high cell adhesion and proliferation activities, because of their low swelling ratios and high sulfonic acid group concentrations. Furthermore, S72-netgels had high FGF-2 activity, because gamma-PGA-S72 retained FGF-2 activity when incorporated into S72-netgels. S72-netgels should be useful as next-generation tissue-engineering material containing FGF-2 activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biocompatible Materials
  • Biodegradation, Environmental
  • Cell Adhesion
  • Cell Line
  • Cell Proliferation
  • Fibroblast Growth Factor 2 / chemistry
  • Fibroblasts / metabolism
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Hydrogels
  • Mice
  • Models, Chemical
  • Polyglutamic Acid / analogs & derivatives*
  • Polyglutamic Acid / chemistry
  • Polyglutamic Acid / pharmacology
  • Polymers / chemistry*
  • Sulfonic Acids / chemistry
  • Temperature
  • Time Factors
  • Tissue Engineering

Substances

  • Biocompatible Materials
  • Hydrogels
  • Polymers
  • Sulfonic Acids
  • poly(glutamic acid)sulfonate
  • Fibroblast Growth Factor 2
  • Polyglutamic Acid
  • Hydrogel, Polyethylene Glycol Dimethacrylate