Abstract
The effects of anxiolytic honokiol derivative, dihydrohonokiol-B (DHH-B), on amyloid beta protein (Abeta(25-35), 10 nM)-induced changes in Cl(-)-ATPase activity, intracellular Cl- concentration ([Cl-]i) and glutamate neurotoxicity were examined in cultured rat hippocampal neurons. DHH-B (10 ng/ml) recovered Abeta-induced decrease in neuronal Cl(-)-ATPase activity without any changes in the activities of Na+/K+-ATPase and anion-insensitive Mg2+-ATPase. A GABA(C) receptor antagonist (1,2,5,6,-tetrahydropyridin-4-yl) methyl-phosphinic acid (TPMPA, 15 microM), inhibited the protective effects of DHH-B on Cl(-)-ATPase activity. DHH-B reduced Abeta-induced elevation of [Cl-]i as assayed using a Cl(-)-sensitive fluorescent dye, and prevented Abeta-induced aggravation of glutamate neurotoxicity. These data suggest that DHH-B exerts the neuroprotective action against Abeta through GABA(C) receptor stimulation.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphatases / metabolism
-
Amyloid beta-Peptides / antagonists & inhibitors
-
Amyloid beta-Peptides / toxicity*
-
Animals
-
Anion Transport Proteins
-
Anti-Anxiety Agents / pharmacology*
-
Biphenyl Compounds / pharmacology*
-
Ca(2+) Mg(2+)-ATPase / metabolism
-
Cell Survival / drug effects
-
Cells, Cultured
-
Chlorides / metabolism
-
Drug Interactions
-
Embryo, Mammalian
-
Glutamic Acid / toxicity
-
Hippocampus / cytology*
-
Lactate Dehydrogenases / metabolism
-
Neurons / drug effects*
-
Rats
-
Rats, Sprague-Dawley
-
Sodium-Potassium-Exchanging ATPase / metabolism
-
Tetrazolium Salts / metabolism
Substances
-
2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt
-
Amyloid beta-Peptides
-
Anion Transport Proteins
-
Anti-Anxiety Agents
-
Biphenyl Compounds
-
Chlorides
-
Tetrazolium Salts
-
dihydrohonokiol
-
Glutamic Acid
-
Lactate Dehydrogenases
-
Adenosine Triphosphatases
-
Ca(2+) Mg(2+)-ATPase
-
anion-sensitive ATPases
-
Sodium-Potassium-Exchanging ATPase