delta-Opioid agonist induced regulation of E2F1 DNA binding activity in NG108-15 cells

Brain Res Mol Brain Res. 2005 May 20;136(1-2):271-4. doi: 10.1016/j.molbrainres.2005.01.004.

Abstract

Activation of opioid receptors have been implicated in the modulation of cell proliferation and the E2F family of transcription factors may play a role in opioid inhibition of DNA synthesis. Gel shift assays and Western blotting of nuclear extracts from NG108-15 cells revealed increased E2F1 DNA binding activity and higher levels of E2F1 following activation of delta-opioid receptors. It is suggested that DADLE-induced regulation of E2F DNA binding activity involves ERKs.

Publication types

  • Comparative Study

MeSH terms

  • Analgesics, Opioid / pharmacology*
  • Animals
  • Blotting, Western / methods
  • Cell Cycle Proteins / metabolism*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • DNA-Binding Proteins / metabolism*
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Electrophoretic Mobility Shift Assay / methods
  • Enkephalin, Leucine-2-Alanine / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • Flavonoids / pharmacology
  • Flow Cytometry / methods
  • Imidazoles / pharmacology
  • Mice
  • Neuroblastoma / pathology
  • Protein Binding / drug effects
  • Pyridines / pharmacology
  • Receptors, Opioid, delta / drug effects*
  • Thymidine / pharmacokinetics
  • Transcription Factors / metabolism*
  • Tritium / pharmacokinetics

Substances

  • Analgesics, Opioid
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • E2f1 protein, mouse
  • Enzyme Inhibitors
  • Flavonoids
  • Imidazoles
  • Pyridines
  • Receptors, Opioid, delta
  • Transcription Factors
  • Tritium
  • Enkephalin, Leucine-2-Alanine
  • SB 203580
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
  • Thymidine