Landscape phage probes for Salmonella typhimurium

Iryna B Sorokulova; Eric V Olsen; I-Hsuan Chen; Ben Fiebor; James M Barbaree; Vitaly J Vodyanoy; Bryan A Chin; Valery A Petrenko

doi:10.1016/j.mimet.2005.02.019

Landscape phage probes for Salmonella typhimurium

J Microbiol Methods. 2005 Oct;63(1):55-72. doi: 10.1016/j.mimet.2005.02.019.

Authors

Iryna B Sorokulova¹, Eric V Olsen, I-Hsuan Chen, Ben Fiebor, James M Barbaree, Vitaly J Vodyanoy, Bryan A Chin, Valery A Petrenko

Affiliation

¹ Department of Pathobiology, Auburn University, 252 Greene Hall, College of Veterinary Medicine, Auburn, AL 36849, USA.

PMID: 15893394
DOI: 10.1016/j.mimet.2005.02.019

Abstract

We selected from landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for S. typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA), and the precipitation test. Interaction of representative probes with S. typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of specific and robust probes for S. typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescent Dyes
Microbiological Techniques / methods*
Peptide Library*
Salmonella typhimurium / chemistry
Salmonella typhimurium / isolation & purification*
Sensitivity and Specificity

Substances

Fluorescent Dyes
Peptide Library