Characterization of a molt-inhibiting hormone (MIH) receptor in the Y-organ of the kuruma prawn, Marsupenaeus japonicus

Ann N Y Acad Sci. 2005 Apr:1040:215-8. doi: 10.1196/annals.1327.027.

Abstract

To characterize a receptor for molt-inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross-linking experiments using radiolabeled recombinant MIH ([(125)I]rMIH). The specific binding of [(125)I]rMIH was found in the membrane fraction of the Y-organ (K(d) = 4.76 x 10(-10) M and B(max) = 5.51 x 10(-12) M). Chemical cross-linking between [(125)I]rMIH and the membrane fraction of the Y-organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.

MeSH terms

  • Animals
  • Endocrine Glands / metabolism*
  • Invertebrate Hormones / chemistry
  • Invertebrate Hormones / isolation & purification
  • Invertebrate Hormones / metabolism*
  • Neuropeptides / chemistry
  • Neuropeptides / isolation & purification
  • Neuropeptides / metabolism*
  • Penaeidae / metabolism*
  • Protein Binding / physiology

Substances

  • Invertebrate Hormones
  • Neuropeptides
  • molt-inhibiting hormone