Abstract
The pig amnion was in vivo intraamniotically infected with E. coli for 10 h at 80-85 d of gestation either with the nonpathogenic O86 strain or enteropathogenic O55 strain. TNF-alpha, IL-10, IL-1beta and IFN-gamma were determined in amniotic fluids by ELISA, the expression of cytokines and some other inflammatory markers was determined by immunohistochemistry. Intraamniotic infection induced high levels of TNF-alpha in amniotic fluids which correlated with bacterial virulence whereas IL-10 was induced only by O86. The IL-1beta level did not increase significantly and was expressed in all infected membranes. IFN-gamma was negligible or absent. TNF-alpha, IL-12p40, calprotectin, HSP65 and gp91phox were found by immunohistochemistry only in amnion membranes infected with the enteropathogenic strain 055.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amnion / immunology*
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Amnion / microbiology
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Animals
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Biomarkers / metabolism*
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Cytokines / metabolism
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Escherichia coli / immunology
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / immunology
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary
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Female
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Inflammation / immunology*
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Interferon-gamma / metabolism
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Interleukin-1 / metabolism
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Interleukin-10 / metabolism
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Pregnancy
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Pregnancy Complications, Infectious / immunology
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Pregnancy Complications, Infectious / microbiology
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Pregnancy Complications, Infectious / veterinary
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Swine
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Swine Diseases / immunology*
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Swine Diseases / microbiology
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Biomarkers
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Cytokines
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Interleukin-1
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interferon-gamma