The complete structure of the core carbohydrate backbone from the LPS of marine halophilic bacterium Pseudoalteromonas carrageenovora type strain IAM 12662T

Carbohydr Res. 2005 Jun 13;340(8):1475-82. doi: 10.1016/j.carres.2005.03.012.

Abstract

The complete novel structure of the components of the core oligosaccharide fraction from the LOS of the halophilic marine bacterium Pseudoalteromonas carrageenovora was characterized. The fully de-acylated lipooligosaccharide was studied by means of compositional analysis, matrix-assisted laser desorption/ionization mass spectrometry and complete (1)H and (13)C and (31)P NMR spectroscopy. The core oligosaccharide is composed by a mixture of species differing for the length of the sugar chain and the phosphorylation pattern: [carbohydrate structure]; see text. All sugars are D-pyranoses. Hep is L-glycero-D-manno-heptose, Kdo is 3-deoxy-D-manno-oct-2-ulosonic acid, P is phosphate, residues and substituents in italic are not stoichiometrically linked.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acylation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / isolation & purification
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Pseudoalteromonas / chemistry*
  • Pseudoalteromonas / classification*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Lipopolysaccharides