Abstract
We have studied the expression of RHOF, a member of the Rho-GTPase family, in an array of lymphoid cells and tissues. Previous microarray studies demonstrated RHOF upregulation in a subset of transformed follicular lymphomas. Real-time quantitative polymerase chain reaction evaluated RHOF expression in lymphocyte subpopulations, and normal and malignant lymphoid tissue. Cells and tissues of B-cell origin expressed higher RHOF levels than their T-cell counterparts. Neoplastic cells and tissues of B-cell origin expressed higher levels of RHOF than their benign cellular counterparts. Relatively elevated levels of RHOF were seen in lymphomas derived from germinal centre origin.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analysis of Variance
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B-Lymphocytes / metabolism
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Cell Line, Tumor
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Cells, Cultured
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Gene Expression
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Germinal Center / immunology
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Humans
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Leukemia / genetics*
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Leukemia, Lymphocytic, Chronic, B-Cell / genetics
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Leukemia, T-Cell / genetics
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Lymphocyte Subsets / metabolism*
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Lymphoid Tissue / metabolism
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Lymphoma / genetics*
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Lymphoma, Large B-Cell, Diffuse / genetics
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Lymphoma, Mantle-Cell / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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T-Lymphocytes / metabolism
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rho GTP-Binding Proteins / genetics*
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rho GTP-Binding Proteins / immunology
Substances
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RHOF protein, human
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rho GTP-Binding Proteins