Filters with a pore size of 0.45 microm have been arbitrarily used for isolating dissolved organic carbon (DOC) in natural waters. This operationally defined DOC fraction often contains heterogeneous organic carbon compounds that may lead to inconsistent results when evaluating trihalomethane formation potential (THMFP). A finer pore size filter provides more homogeneous DOC properties and enables a better characterization of organic matter. In this study, we examined the effects of filter pore size (1.2, 0.45, 0.1 and 0.025 microm) on characterizing total organic carbon, ultra-violet absorbance at 254 nm (UV(254)) and THMFP of water extracts from a mineral and organic soil in the Sacramento-San Joaquin Delta, California. Results showed that the majority of water extractable organic carbon (WEOC) from these soils was smaller than 0.025 microm, 85% and 57% in organic and mineral soils, respectively. A high proportion of colloidal organic carbon (COC) in mineral soil extracts caused water turbidity and resulted in an abnormally high UV(254) in 1.2 and 0.45 microm filtrates. The reactivity of organic carbon fractions in forming THM was similar for the two soils, except that COC from the mineral soil was about half that of others. To obtain a more homogeneous solution for characterizing THM precursors, we recommend a 0.1 microm or smaller pore-size filter, especially for samples with high colloid concentrations.