Abstract
Corticotropin releasing hormone (CRH) is a major regulator of the stress response. This study examined whether CRH regulates interleukin-18 expression on microglia, BV2. Our data show that CRH enhanced IL-18 expression and significantly induced the secretion of functional IL-18 protein. Furthermore, CRH induced IL-18 production could be blocked by N-acetyl-L-cystein (NAC), which suggests that reactive oxygen intermediates (ROI) may be involved in regulating IL-18. Indeed, it was also found that CRH increased the generation of ROI. Taken together, these results indicate that CRH is an important mediator that regulates IL-18 expression in the brain during stress.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Corticotropin-Releasing Hormone / pharmacology*
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Gene Expression Regulation / drug effects*
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Interferon-gamma / biosynthesis
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Interferon-gamma / metabolism
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Interleukin-18 / genetics
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Interleukin-18 / metabolism*
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Mice
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Mice, Inbred C57BL
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Microglia / drug effects*
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Microglia / metabolism*
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RNA, Messenger / genetics
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Reactive Oxygen Species / metabolism
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Receptors, Corticotropin-Releasing Hormone / genetics
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Signal Transduction / drug effects
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Titrimetry
Substances
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Interleukin-18
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RNA, Messenger
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Reactive Oxygen Species
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Receptors, Corticotropin-Releasing Hormone
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Interferon-gamma
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Corticotropin-Releasing Hormone