Molecular cloning and in vitro evaluation of an infectious simian-human immunodeficiency virus containing env of a primary Chinese HIV-1 subtype C isolate

J Med Primatol. 2005 Apr;34(2):101-7. doi: 10.1111/j.1600-0684.2005.00098.x.

Abstract

Human immunodeficiency virus (HIV) clade C is the most prevalent subtype and accounts for approximately 50% of all HIV infections worldwide. In China, the prevalent HIV strains are B'/C subtypes, in which the envelope belongs to subtype C. To evaluate potential AIDS vaccines targeting Chinese viral strains in non-human primate models, we constructed an infectious simian-human immunodeficiency virus (SHIV) that expresses most of the envelope of a primary HIV strain, which was isolated from a HIV-positive intravenous drug user from XinJiang province in China. The resulting chimeric SHIV-XJ02170 was infectious in human, rhesus monkey and cynomolgus monkey peripheral blood mononuclear cells (PBMC) and used CCR5 exclusively as coreceptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • China
  • Cloning, Molecular*
  • Genetic Engineering
  • HIV Envelope Protein gp160 / genetics
  • HIV-1 / genetics*
  • HIV-1 / growth & development
  • HIV-1 / pathogenicity
  • Humans
  • Leukocytes, Mononuclear / virology
  • Macaca fascicularis
  • Macaca mulatta
  • Organisms, Genetically Modified*
  • Phylogeny
  • RNA, Viral / analysis
  • Receptors, CCR5 / physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Simian Immunodeficiency Virus / genetics*
  • Simian Immunodeficiency Virus / growth & development
  • Simian Immunodeficiency Virus / pathogenicity
  • Virus Replication

Substances

  • HIV Envelope Protein gp160
  • RNA, Viral
  • Receptors, CCR5