The study showed that different ecological environment and cultivation system in various ecological regions of Zhejiang Province resulted in some different physiological characteristics of nitrogen nutrition and stress-resistance, especially in the aspect of NO3(-)-N and NH4+-N concentrations, between film-mulched and conventional flooded rice. Owing to the heat stress in Hangjiahu plain, the NO3(-)-N concentration of film-mulched rice decreased to some extent, but NH4+-N concentration increased markedly at tillering, jointing and booting stages, compared to conventional flooded rice. In Jinqu basin, the NO3(-)-N concentration of film-mulched rice at booting stage was higher, while the NH4+-N concentration in its roots was notably lower than those of conventional flooded rice, with NH4+-N concentration in its basal stems and leaves somewhat increased. Generally, the glutamine synthetase (GS) and nitrate reductase (NR) activities in film-mulched rice leaves were enhanced at booting stage, while malondiadehyde (MDA), soluble sugar (SS) and proline (Pro) concentrations had little changes. In conclusion, film-mulched cultivation was beneficial to the rice growth and its high yielding.