On the medium MS added the right amount of 2,4-D and LFS (Angustmycin) and cultured under dark condition, the callus from stem segments of Panax notoginseng could induce a lot of embryoids. In 2-3 months, the ratio of embryoid formation reached about 90%. Then transplanted on MS + 2,4-D 1.5 mg/L + LFS 2 mg/L and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets.