Involvement of Saccharomyces cerevisiae Pdr5p ATP-binding cassette transporter in calcium homeostasis

Andreea Cristina Tutulan-Cunita; Tomoko Ohnishi; Masaki Mizunuma; Dai Hirata; Tokichi Miyakawa

doi:10.1271/bbb.69.857

Involvement of Saccharomyces cerevisiae Pdr5p ATP-binding cassette transporter in calcium homeostasis

Biosci Biotechnol Biochem. 2005 Apr;69(4):857-60. doi: 10.1271/bbb.69.857.

Authors

Andreea Cristina Tutulan-Cunita¹, Tomoko Ohnishi, Masaki Mizunuma, Dai Hirata, Tokichi Miyakawa

Affiliation

¹ Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.

PMID: 15849433
DOI: 10.1271/bbb.69.857

Abstract

Deletion of PDR5 gene (Deltapdr5) in Saccharomyces cerevisiae led to increased resistance to calcium. The cellular Ca2+ level in the presence of high calcium as estimated by reporter assay in Deltapdr5 cells was significantly lower than that in wild-type cells. Membrane Pdr5p levels diminished rapidly during incubation with high calcium in a manner dependent on calcineurin and Pep4p, suggesting a feedback regulatory mechanism for Pdr5p abundance.

MeSH terms

ATP-Binding Cassette Transporters / genetics
ATP-Binding Cassette Transporters / metabolism*
Calcium / metabolism*
Calcium / pharmacology
Gene Expression Regulation, Fungal / drug effects
Homeostasis*
Mutation / genetics
Saccharomyces cerevisiae / drug effects
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*

Substances

ATP-Binding Cassette Transporters
PDR5 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Calcium