Abstract
We previously reported that p38 MAP kinase takes part in thrombin-induced HSP27 phosphorylation in aortic smooth muscle A10 cells. In the present study, we investigated whether Akt is involved in the phosphorylation of HSP27 and the role of adenylyl cyclase-cAMP system. Thrombin time-dependently induced the phosphorylation of heat shock protein 27 (HSP27) and Akt in aortic smooth muscle A10 cells. SB203580, a p38 MAP kinase inhibitor, significantly suppressed the thrombin-induced phosphorylation of Akt and the Akt inhibitor suppressed the phosphorylation of HSP27. Furthermore, the thrombin-induced phosphorylation of HSP27, p38 MAP kinase and Akt were decreased by dibutyryl-cAMP (DBcAMP). These results strongly suggest that Akt functions the thrombin-induced phosphorylation of HSP27 at a point downstream from p38 MAP kinase in aortic smooth muscle cells and the adenylyl cyclase-cAMP system is upstream regulator of the HSP27 phosphorylation in these cells.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenylyl Cyclases / drug effects
-
Adenylyl Cyclases / physiology
-
Animals
-
Aorta / cytology
-
Bucladesine / pharmacology
-
Cells, Cultured
-
Colforsin / pharmacology
-
Cyclic AMP / pharmacology
-
Heat-Shock Proteins / metabolism*
-
Imidazoles / pharmacology
-
Muscle, Smooth, Vascular / cytology
-
Muscle, Smooth, Vascular / enzymology*
-
Phosphorylation / drug effects
-
Protein Kinase Inhibitors / pharmacology
-
Protein Serine-Threonine Kinases / antagonists & inhibitors
-
Protein Serine-Threonine Kinases / metabolism
-
Protein Serine-Threonine Kinases / physiology*
-
Proto-Oncogene Proteins / antagonists & inhibitors
-
Proto-Oncogene Proteins / metabolism
-
Proto-Oncogene Proteins / physiology*
-
Proto-Oncogene Proteins c-akt
-
Pyridines / pharmacology
-
Rats
-
Thrombin / physiology*
-
p38 Mitogen-Activated Protein Kinases / physiology*
Substances
-
Heat-Shock Proteins
-
Imidazoles
-
Protein Kinase Inhibitors
-
Proto-Oncogene Proteins
-
Pyridines
-
Colforsin
-
Bucladesine
-
Cyclic AMP
-
Akt1 protein, rat
-
Protein Serine-Threonine Kinases
-
Proto-Oncogene Proteins c-akt
-
p38 Mitogen-Activated Protein Kinases
-
Thrombin
-
Adenylyl Cyclases
-
2-(4-nitrophenyl)-4-(4-fluorophenyl)-5-(4-pyridinyl)-1H-imidazole
-
SB 203580