Abstract
An ethyl acetate extract of Artemisia herba-alba was partitioned by HPLC in 10 fractions that were tested in the [(3)H]-flumazenil radioligand assay, for affinity to the GABA(A)-benzodiazepine receptor. Two fractions showed activity from which hispidulin and cirsilineol were isolated. The structures were confirmed by (1)H NMR. The IC(50) values were 8 microM for hispidulin and 100 microM for cirsilineol.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Artemisia / chemistry*
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Chromatography, High Pressure Liquid
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Flavones / isolation & purification
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Flavones / pharmacology
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Flavonoids / pharmacology*
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Flumazenil / pharmacology
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GABA Agonists / isolation & purification
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GABA Agonists / pharmacology
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GABA Antagonists / isolation & purification
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GABA Antagonists / pharmacology
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GABA Modulators / pharmacology
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Lebanon
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Magnetic Resonance Spectroscopy
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Plant Extracts / chemistry
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Radioligand Assay
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Receptors, GABA-A / drug effects*
Substances
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Flavones
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Flavonoids
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GABA Agonists
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GABA Antagonists
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GABA Modulators
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Plant Extracts
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Receptors, GABA-A
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Flumazenil
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cirsilineol
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hispidulin