Two flavonoids from Artemisia herba-alba Asso with in vitro GABAA-benzodiazepine receptor activity

J Ethnopharmacol. 2005 May 13;99(1):145-6. doi: 10.1016/j.jep.2005.01.031.

Abstract

An ethyl acetate extract of Artemisia herba-alba was partitioned by HPLC in 10 fractions that were tested in the [(3)H]-flumazenil radioligand assay, for affinity to the GABA(A)-benzodiazepine receptor. Two fractions showed activity from which hispidulin and cirsilineol were isolated. The structures were confirmed by (1)H NMR. The IC(50) values were 8 microM for hispidulin and 100 microM for cirsilineol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Artemisia / chemistry*
  • Chromatography, High Pressure Liquid
  • Flavones / isolation & purification
  • Flavones / pharmacology
  • Flavonoids / pharmacology*
  • Flumazenil / pharmacology
  • GABA Agonists / isolation & purification
  • GABA Agonists / pharmacology
  • GABA Antagonists / isolation & purification
  • GABA Antagonists / pharmacology
  • GABA Modulators / pharmacology
  • Lebanon
  • Magnetic Resonance Spectroscopy
  • Plant Extracts / chemistry
  • Radioligand Assay
  • Receptors, GABA-A / drug effects*

Substances

  • Flavones
  • Flavonoids
  • GABA Agonists
  • GABA Antagonists
  • GABA Modulators
  • Plant Extracts
  • Receptors, GABA-A
  • Flumazenil
  • cirsilineol
  • hispidulin