Saturation transfer difference measurements with SU(VAR)3-9 and S-adenosyl-L-methionine

Biochemistry. 2005 Apr 26;44(16):6208-13. doi: 10.1021/bi047749x.

Abstract

Saturation transfer difference NMR measurements were performed to investigate the interaction of S-adenosyl-l-methionine (AdoMet) with SU(VAR)3-9 from Drosophila melanogaster. SU(VAR)3-9 has a SET domain and plays an important role in methylation of lysine-9 of histone H3 which results in gene silencing. We determined the binding epitope of AdoMet and compared it with a crystal structure of another SET protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Drosophila Proteins / chemistry*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • In Vitro Techniques
  • Kinetics
  • Methylation
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Conformation
  • Repressor Proteins / chemistry*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • S-Adenosylmethionine / chemistry
  • S-Adenosylmethionine / metabolism*

Substances

  • Drosophila Proteins
  • Repressor Proteins
  • Su(var)3-9 protein, Drosophila
  • S-Adenosylmethionine