Abstract
The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1 cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis Proteins / genetics
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Biotechnology
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Cell Differentiation
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Chromatography, High Pressure Liquid
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DNA, Complementary / metabolism
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Dose-Response Relationship, Drug
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Flavonoids / metabolism
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Free Radicals
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Gene Expression Regulation, Plant
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Genes, Plant
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Genetic Engineering / methods*
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Models, Chemical
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Mutation
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Pancreatitis-Associated Proteins
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Plants, Genetically Modified*
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Proanthocyanidins / metabolism
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Promoter Regions, Genetic
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Proto-Oncogene Proteins c-myb / chemistry
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Recombinant Fusion Proteins / chemistry
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Seeds
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Spectrometry, Mass, Electrospray Ionization
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Time Factors
Substances
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Arabidopsis Proteins
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DNA, Complementary
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Flavonoids
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Free Radicals
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Pancreatitis-Associated Proteins
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Proanthocyanidins
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Proto-Oncogene Proteins c-myb
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REG3A protein, human
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Recombinant Fusion Proteins
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proanthocyanidin