Abstract
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics*
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Bacteriophage HK022 / enzymology*
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Binding Sites / genetics
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Blotting, Northern
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Blotting, Southern
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Cell Nucleus / metabolism
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DNA, Plant / genetics
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DNA, Plant / metabolism
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Gene Expression Regulation, Enzymologic
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Immunoblotting
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Integrases / genetics*
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Integrases / metabolism
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Microscopy, Fluorescence
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Nicotiana / genetics
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Plants, Genetically Modified
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Recombination, Genetic / genetics*
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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DNA, Plant
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Recombinant Fusion Proteins
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Viral Proteins
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Green Fluorescent Proteins
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Integrases