Tethered or adsorbed supported lipid bilayers in nanotubes characterized by deuterium magic angle spinning NMR spectroscopy

Langmuir. 2005 Apr 12;21(8):3226-8. doi: 10.1021/la0469147.

Abstract

2H solid-state NMR experiments were performed under magic angle spinning on lipid bilayers oriented into nanotubes arrays, as a new method to assess the geometrical arrangement of the lipids. Orientational information is obtained from the intensities of the spinning sidebands. The lipid bilayers are formed by fusion of small unilamellar vesicles of DMPC-d54 inside a nanoporous anodic aluminum oxide, either by direct adsorption on the support or by tethering through a streptavidin/biotin linker. The results support that the quality of the lipid bilayers alignment is clearly in favor of the tethering rather than an adsorbed strategy.

Publication types

  • Letter

MeSH terms

  • Adsorption
  • Aluminum Oxide / chemistry
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Biotin / chemistry
  • Deuterium / chemistry*
  • Dimyristoylphosphatidylcholine / chemistry
  • Lipid Bilayers / chemistry*
  • Magnetic Resonance Spectroscopy / instrumentation
  • Magnetic Resonance Spectroscopy / methods*
  • Nanotubes / chemistry*
  • Porosity
  • Streptavidin / chemistry

Substances

  • Lipid Bilayers
  • Biotin
  • Streptavidin
  • Deuterium
  • Aluminum Oxide
  • Dimyristoylphosphatidylcholine