Abstract
Objective:
To study the specificity of prostate-specific membrane antigen (PSMA) promoter in controlling gene expression.
Methods:
PSMA promoter gene was amplified with PCR, and then the promoter gene was cloned into the vector pEGFP-1 to construct a recombinant plasmid, which was transfected into different cell lines such as LNcap, PC-3, MCF-7, A549. Green fluorescent protein (GFP) expression was observed.
Results:
The recombinant plasmid constructed, and PSMA promoter uniquely showed modulating activity in PSMA positive cell line.
Conclusion:
PSMA promoter possesses PSMA positive cell specificity, as well as prostatic tissue specificity. PSMA promoter may have the potential for use in targeted gene therapy of prostate adenocarcinoma.
MeSH terms
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Adenocarcinoma / therapy
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Antigens, Neoplasm / biosynthesis
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Antigens, Neoplasm / genetics*
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Antigens, Surface / biosynthesis
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Antigens, Surface / genetics*
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Enhancer Elements, Genetic / genetics
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Gene Expression Regulation, Neoplastic
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Genes, Reporter
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Glutamate Carboxypeptidase II / biosynthesis
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Glutamate Carboxypeptidase II / genetics*
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Green Fluorescent Proteins / genetics
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Humans
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Male
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Molecular Sequence Data
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Plasmids / genetics
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Promoter Regions, Genetic
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Prostate / immunology*
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Prostate / metabolism
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Prostatic Neoplasms / therapy
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
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Transfection
Substances
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Antigens, Neoplasm
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Antigens, Surface
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RNA, Messenger
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Recombinant Proteins
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Green Fluorescent Proteins
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FOLH1 protein, human
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Glutamate Carboxypeptidase II