Objective: To investigate the biological effects of the new nano-hydroxyapatite/polyamide 66 biological composites (nHA-PA66) on the dental pulp cells.
Methods: After interaction with the nHA-PA66 eluate, the growth, proliferation, and function of the in vitro cultured human dental pulp cells were studied by cell culture technique, inverted phase-contrast microscope observation, MTT assay, flow cytometry, ALP activity assay and quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) analysis.
Results: The cultured pulp cells grew well and showed no morphological variation. Moreover, this material had no negative effects on the proliferation, cell cycle, ALP activity and the expression of dentin sialophosphoprotein (DSPP) mRNA of the pulp cells.
Conclusion: As a new nano-biomaterial, nHA-PA66 has good biocompatibility to the pulp cells, but no obvious bioactivity.