Abstract
In this study, we investigated the interaction between lipopolysaccharide (LPS) and lead (Pb) and the involvement of tumor necrosis factor-alpha (TNF-alpha) and oxidative stress in Pb-plus-LPS (Pb/LPS)-induced liver damage in rats. Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), TNF-alpha, nitric oxide (NO), and lipid peroxidation (LPO) were determined in rats treated with Pb and/or LPS. Pb ranging from 0 to 15 mg/kg dose dependently increased AST, ALT, NO, or LPO in LPS-treated rats. Pretreatment with iNOS inhibitor 1400W reduced NO, LPO, TNF-alpha, AST, and ALT in Pb/LPS-treated rats. Thus, Pb increased LPS-induced liver damage, which might be associated with increased NO-initiated oxidative stress and TNF-alpha in rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine Transaminase / blood
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Amidines / pharmacology
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Animals
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Aspartate Aminotransferases / blood
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Benzylamines / pharmacology
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Lead / metabolism
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Lead / pharmacology
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Lead / toxicity*
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Lipid Peroxidation
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Lipopolysaccharides / blood
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Lipopolysaccharides / pharmacology
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Lipopolysaccharides / toxicity*
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Liver / drug effects*
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Liver / injuries*
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Male
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / metabolism*
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type II
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Nitrites / blood
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Organometallic Compounds / pharmacology
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Oxidative Stress*
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Rats
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Rats, Wistar
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Tumor Necrosis Factor-alpha / biosynthesis*
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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Amidines
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Benzylamines
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Enzyme Inhibitors
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Lipopolysaccharides
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N-(3-(aminomethyl)benzyl)acetamidine
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Nitrites
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Organometallic Compounds
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Tumor Necrosis Factor-alpha
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Lead
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Nitric Oxide
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, rat
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Aspartate Aminotransferases
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Alanine Transaminase
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lead acetate