Structure of the O-polysaccharide from the Azospirillum lipoferum Sp59b lipopolysaccharide

Yuliya P Fedonenko; Olga N Konnova; George V Zatonsky; Svetlana A Konnova; Nina A Kocharova; Evelina L Zdorovenko; Vladimir V Ignatov

doi:10.1016/j.carres.2005.01.042

Structure of the O-polysaccharide from the Azospirillum lipoferum Sp59b lipopolysaccharide

Carbohydr Res. 2005 May 2;340(6):1259-63. doi: 10.1016/j.carres.2005.01.042.

Authors

Yuliya P Fedonenko¹, Olga N Konnova, George V Zatonsky, Svetlana A Konnova, Nina A Kocharova, Evelina L Zdorovenko, Vladimir V Ignatov

Affiliation

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.

PMID: 15797145
DOI: 10.1016/j.carres.2005.01.042

Abstract

A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant-growth-promoting bacterium Azospirillum lipoferum Sp59b. On the basis of sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy, including a NOESY experiment, the following structure of the branched hexasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text].

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum lipoferum / chemistry*
Carbohydrate Sequence
Magnetic Resonance Spectroscopy
Methylation
O Antigens / chemistry*

Substances

O Antigens