Single DNA molecule detection using nanopipettes and nanoparticles

Nano Lett. 2005 Feb;5(2):403-7. doi: 10.1021/nl0480464.

Abstract

Single DNA molecules labeled with nanoparticles can be detected by blockades of ionic current as they are translocated through a nanopipette tip formed by a pulled glass capillary. The nanopipette detection technique can provide not only tools for detection and identification of single DNA and protein molecules but also deeper insight and understanding of stochastic interactions of various biomolecules with their environment.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • DNA / analysis*
  • DNA / chemistry*
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Gold / analysis
  • Gold / chemistry
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Nanotechnology / instrumentation*
  • Nanotechnology / methods
  • Nanotubes / analysis
  • Nanotubes / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Gold
  • DNA