Abstract
A new natural TEM derivative with extended-spectrum beta-lactamase activity, TEM-134, was identified in a ceftazidime-resistant clinical isolate of Citrobacter koseri. Compared to TEM-1, TEM-134 contains the following mutations: Q39K, E104K, R164H, and G238S. The bla(TEM-134) gene was not transferable by conjugation and, apparently, was chromosomally encoded. Expression studies with Escherichia coli revealed efficient cefotaximase and ceftazidimase activity for TEM-134.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Ceftazidime / pharmacology
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Cephalosporin Resistance
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Citrobacter koseri / drug effects
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Citrobacter koseri / enzymology*
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Citrobacter koseri / genetics
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Drug Resistance, Bacterial
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Enterobacteriaceae Infections / microbiology
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Sequence Analysis, DNA
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beta-Lactamases / classification*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Ceftazidime
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beta-Lactamases