Inserting foreign peptides into the major coat protein of bacteriophage M13

FEBS Lett. 1992 Apr 27;301(3):322-4. doi: 10.1016/0014-5793(92)80267-k.

Abstract

Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B-proteins have the same contour length and the same number of B-protein molecules as virions with natural B-proteins. It was shown that for one of hybrid B-proteins the position of the processing site had changed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteriophages / genetics*
  • Bacteriophages / pathogenicity
  • Base Sequence
  • Capsid / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed*
  • Peptides / genetics*
  • Plasmids

Substances

  • Peptides