Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway

Kristin E Burns; Yun Xiang; Cynthia L Kinsland; Fred W McLafferty; Tadhg P Begley

doi:10.1021/ja042792t

Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway

J Am Chem Soc. 2005 Mar 23;127(11):3682-3. doi: 10.1021/ja042792t.

Authors

Kristin E Burns¹, Yun Xiang, Cynthia L Kinsland, Fred W McLafferty, Tadhg P Begley

Affiliation

¹ Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

PMID: 15771487
DOI: 10.1021/ja042792t

Abstract

The substrates for Bacillus subtilis PLP synthase (YaaD and YaaE) are identified, and the first reconstitution of PLP biosynthesis using this pathway is described. Three partial reactions catalyzed by YaaD are also identified.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Anthranilate Synthase / metabolism
Bacillus subtilis / enzymology
Bacillus subtilis / metabolism
Bacterial Proteins / metabolism
Fourier Analysis
Glutaminase / metabolism
Nitrogenous Group Transferases / metabolism
Pyridoxal Phosphate / biosynthesis*
Spectrometry, Mass, Electrospray Ionization

Substances

Bacterial Proteins
Pyridoxal Phosphate
Nitrogenous Group Transferases
Glutaminase
Anthranilate Synthase
anthranilate synthase, glutamine amidotransferase subunit

Abstract

Publication types

MeSH terms

Substances

Grants and funding