Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis

Holger Christian Scholz; Angela Witte; Herbert Tomaso; Sascha Al Dahouk; Heinrich Neubauer

doi:10.1016/j.femsle.2005.02.005

Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis

FEMS Microbiol Lett. 2005 Mar 15;244(2):347-52. doi: 10.1016/j.femsle.2005.02.005.

Authors

Holger Christian Scholz¹, Angela Witte, Herbert Tomaso, Sascha Al Dahouk, Heinrich Neubauer

Affiliation

¹ Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, D-80937 Munich, Germany. holger1scholz@bundeswehr.org

PMID: 15766789
DOI: 10.1016/j.femsle.2005.02.005

Abstract

Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence - and by restriction fragment length polymorphism analysis of the recombinase A gene (recA-PCR-RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472(T) allowed the specific amplification of a 1040bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. HindII-PstI-recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level.

MeSH terms

Bacterial Typing Techniques
Chromobacterium / classification*
Chromobacterium / genetics
DNA, Bacterial / analysis*
Genotype
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length*
Rec A Recombinases / genetics*

Substances

DNA, Bacterial
Rec A Recombinases