Abstract
For humoral immunization, it may be possible to make effective and safe peptide vaccines for various diseases by selection of proper B-cell epitopes. However, a lack of T-cell epitopes on short peptides, such as those associated with major histocompatibility complex (MHC)-restriction, is a major problem for peptide vaccine development. We propose a solution for the design of peptide vaccines that involves induction of broadly reactive T-cell epitopes via agretopes. The strategy involves positioning multi-agretope type peptides on the N-terminal side of a di-lysine linker and B-cell epitopes on the C-terminal side. The addition of the arginine-glysine-aspartate (RGD)-motif to the N terminus of the peptide enhances its immunogenicity, and enables nasal immunization without adjuvants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Drug Design
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Epitopes, B-Lymphocyte / chemistry
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Epitopes, B-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / chemistry
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Epitopes, T-Lymphocyte / genetics
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H-2 Antigens
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / chemical synthesis
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Influenza Vaccines / chemistry
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Influenza Vaccines / genetics
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Molecular Sequence Data
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Oligopeptides / chemistry
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Vaccines, Subunit / administration & dosage
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Vaccines, Subunit / chemical synthesis
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Vaccines, Subunit / chemistry*
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Vaccines, Subunit / genetics
Substances
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Epitopes, B-Lymphocyte
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Epitopes, T-Lymphocyte
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H-2 Antigens
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Influenza Vaccines
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Oligopeptides
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Vaccines, Subunit
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arginyl-glycyl-aspartic acid