Biosensor signal amplification of vesicles functionalized with glycolipid for colorimetric detection of Escherichia coli

J Colloid Interface Sci. 2005 Apr 1;284(1):114-9. doi: 10.1016/j.jcis.2004.10.003.

Abstract

A supramolecular assembly of phospholipid-polymerized diacetylene vesicles functionalized with glycolipid can provide a molecular recognition function. The Escherichia coli-glycolipid binding event leads to a visible color change from blue to red, readily seen with the naked eye and quantified by absorption spectroscopy. The biosensor signal is amplified through a suitable increase of phospholipid content in the mixed lipid vesicles and pH of aqueous solutions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylene / analogs & derivatives
  • Acetylene / chemistry
  • Bacteriological Techniques / methods
  • Biosensing Techniques / methods*
  • Colorimetry / methods
  • Dimyristoylphosphatidylcholine / chemistry
  • Escherichia coli / isolation & purification*
  • Fatty Acids, Unsaturated / chemistry
  • Glycolipids / chemistry*
  • Hydrogen-Ion Concentration
  • Liposomes / chemistry*
  • Mannose / chemistry
  • Microscopy, Electron, Transmission
  • Oligosaccharides / chemistry
  • Photochemistry
  • Polyacetylene Polymer
  • Polymers / chemistry
  • Polyynes
  • Spectrophotometry
  • Ultraviolet Rays

Substances

  • 10, 12-pentacosadiynoic acid
  • Fatty Acids, Unsaturated
  • Glycolipids
  • Liposomes
  • Oligosaccharides
  • Polyacetylene Polymer
  • Polymers
  • Polyynes
  • polydiacetylene
  • Acetylene
  • Mannose
  • Dimyristoylphosphatidylcholine