An indirect quantitative method for the assessment of angiogenesis in human tissues

Christine Lawson; Catherine Herrmann-Lavoie; André Lemay; Walid Mourad; Ali Akoum

doi:10.1016/j.fertnstert.2004.10.031

An indirect quantitative method for the assessment of angiogenesis in human tissues

Fertil Steril. 2005 Mar;83(3):789-92. doi: 10.1016/j.fertnstert.2004.10.031.

Authors

Christine Lawson¹, Catherine Herrmann-Lavoie, André Lemay, Walid Mourad, Ali Akoum

Affiliation

¹ Laboratoire d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-François d'Assise, Université Laval, Québec, Québec, Canada.

PMID: 15749521
DOI: 10.1016/j.fertnstert.2004.10.031

Abstract

We set up an ELISA that measures the concentration of von Willebrand factor (vWF) in human endometrial tissue and found a significant correlation with the mean vessel counts in vWF- and CD31-immunostained tissue sections. This ELISA allows an objective and quantitative evaluation of the vascular state in the endometrium and could be used as a method to evaluate the angiogenic state in other tissues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Endometrium / metabolism*
Enzyme-Linked Immunosorbent Assay / methods*
Female
Humans
Immunohistochemistry
Neovascularization, Physiologic / physiology*
Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
von Willebrand Factor / metabolism*

Substances

Platelet Endothelial Cell Adhesion Molecule-1
von Willebrand Factor