Influence of amino acid substitutions in the leader peptide on maturation and secretion of mesentericin Y105 by Leuconostoc mesenteroides

J Bacteriol. 2005 Mar;187(6):2218-23. doi: 10.1128/JB.187.6.2218-2223.2005.

Abstract

By site-specific mutagenesis, the hydrophobic conserved amino acids and the C-terminal GG doublet of the leader peptide of pre-mesentericin Y105 were demonstrated to be critical for optimal secretion of mesentericin Y105, as well as for the maturation of the pre-bacteriocin by the protease portion of the ABC transporter MesD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Bacteriocins / chemistry
  • Bacteriocins / genetics*
  • Bacteriocins / metabolism*
  • Cytoplasm / metabolism
  • Hydrophobic and Hydrophilic Interactions
  • Leuconostoc / genetics*
  • Leuconostoc / metabolism*
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Protein Structure, Tertiary

Substances

  • Bacteriocins
  • mesentericin Y105 protein, Leuconostoc mesenteroides