Abstract
We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Kinetics
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Peptidoglycan / chemistry
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Peptidoglycan / metabolism
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Peptidoglycan Glycosyltransferase / genetics
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Peptidoglycan Glycosyltransferase / metabolism*
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Staphylococcus aureus / enzymology*
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Staphylococcus aureus / genetics
Substances
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Peptidoglycan
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Peptidoglycan Glycosyltransferase