Stepwise gradient of buffer concentration for capillary electrochromatography of peptides on sulfonated naphthalimido-modified silyl silica gel

Kaname Ohyama; Mitsuhiro Wada; Naoya Kishikawa; Yoshihito Ohba; Kenichiro Nakashima; Naotaka Kuroda

doi:10.1016/j.chroma.2004.12.052

Stepwise gradient of buffer concentration for capillary electrochromatography of peptides on sulfonated naphthalimido-modified silyl silica gel

J Chromatogr A. 2005 Feb 4;1064(2):255-9. doi: 10.1016/j.chroma.2004.12.052.

Authors

Kaname Ohyama¹, Mitsuhiro Wada, Naoya Kishikawa, Yoshihito Ohba, Kenichiro Nakashima, Naotaka Kuroda

Affiliation

¹ Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.

PMID: 15739894
DOI: 10.1016/j.chroma.2004.12.052

Abstract

The advantage of using a stepwise gradient of buffer concentration in CEC was demonstrated with the mixed-mode stationary phase, 3-(4-sulfo-1,8-naphthalimido)propyl-modified silyl silica gel (SNAIP). Before the application of a stepwise gradient, the effect of buffer concentration on the separations of six peptides and tryptic digests was investigated. Bubble formation caused by Joule heating at currents up to 95 microA was successfully suppressed by using SNAIP column even without pressurization, which contributed to a stepwise gradient of buffer concentration. Utilizing the stepwise gradient improved and shortened the separation of six peptides as compared to the separation under an isocratic elution.

MeSH terms

Buffers*
Chromatography, Micellar Electrokinetic Capillary / methods*
Silica Gel
Silicon Dioxide / chemistry*

Substances

Buffers
Silica Gel
Silicon Dioxide