Several neuroimaging studies have consistently demonstrated the critical involvement of prefrontal cortices and medial temporal lobes during long-term encoding. While the contribution of prefrontal lobes to working memory is well established, the role of the MTL structures remains controversial. To address this issue, we registered the neuromagnetic brain patterns of eight adult volunteers while they performed two working memory tasks (verbal and spatial) using magnetoencephalography (MEG). MEG recordings can provide real-time measures of brain activity, thus allowing detailed tracking of the time-course of brain activation during the encoding phase. We detected sustained and material-specific activity on the MTLs during the encoding phase of a working memory task, based on verbal and spatial information. Two peaks of activation were noted in the left MTL during word encoding in two non-consecutive time periods (500-600 ms and 700-800 ms after stimulus onset). Right MTL laterality was found for encoding locations when we collapsed activity sources in a wider time period (400-800 ms). In addition, we provided the spatiotemporal profiles of what seems to be two different brain circuits specific for each type of material. Finally, following an emerging conceptualization of working memory, we hypothesized that encoding processes mediated by the MTL to long-term memory would also apply to working memory.