The objective of this study was to determine the species and distribution of ticks infesting domestic dogs in southern Mozambique. To this end 89 collections were taken from dogs and the geographic coordinates of the localities at which they were made noted. Fifty-eight of these collections were from animals in the city of Maputo and 16 from animals at rural settlements close to Maputo. A further 15 collections were made from dogs in rural environments north of Maputo. Ten ixodid tick species, of which one was only identified to genus level, were recovered. Rhipicephalus sanguineus was the most numerous of the ten species, and its prevalence and intensity of infestation were significantly higher on city dogs than on rural dogs (P < 0.01), whereas the converse was true for Haemaphysalis leachi (P < 0.01). Including the city of Maputo, the exact localities at which nine tick species were collected, were recorded.