[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne Borreliosis (Lyme disease). Communication 2. Comparative study of recombinant enzyme immunoassay test-systems (rELISA) for serological diagnosis of ixode tick-borne Borreliosis]

I N Manzeniuk; M S Vorob'eva; N M Nikitiuk; E A Arumova; L P Anan'eva; S G Baranova; T G Viatkina; A V Masiago; A A Kozarenko; Iu A Andreĭchuk

[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne Borreliosis (Lyme disease). Communication 2. Comparative study of recombinant enzyme immunoassay test-systems (rELISA) for serological diagnosis of ixode tick-borne Borreliosis]

Antibiot Khimioter. 2004;49(8-9):25-8.

[Article in Russian]

Authors

I N Manzeniuk, M S Vorob'eva, N M Nikitiuk, E A Arumova, L P Anan'eva, S G Baranova, T G Viatkina, A V Masiago, A A Kozarenko, Iu A Andreĭchuk

PMID: 15727142

Abstract

One foreign and two Russian recombinant enzyme immunoassay test-systems were comparatively investigated under conditions of an encoded experiment. Sensitivity in the experiment with the Russian test-systems Borreliosis-ELISA-IgG and Lyme Best was 63.8 and 68.8% respectively. As for the test-system Borrelia IgG Recombinant, it was 47.5%. All the test-systems were highly specific (94.4 to 99.5%). The test-systems Lyme Best and Borrelia IgG Recombinant revealed partial cross reactions with sera from patients with systemic lupus erythematosus and leptospirosis.

Publication types

Comparative Study
English Abstract

MeSH terms

Antibodies, Bacterial / blood
Antigens, Bacterial / immunology
Borrelia burgdorferi Group / immunology*
Cross Reactions
Enzyme-Linked Immunosorbent Assay / methods
Humans
Immunoglobulin G / blood
Lyme Disease / blood
Lyme Disease / diagnosis*
Reagent Kits, Diagnostic
Recombinant Proteins / immunology
Sensitivity and Specificity
Serologic Tests / methods

Substances

Antibodies, Bacterial
Antigens, Bacterial
Immunoglobulin G
Reagent Kits, Diagnostic
Recombinant Proteins