Removal of contaminating DNA from commercial nucleic acid extraction kit reagents

Tamimount Mohammadi; Henk W Reesink; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul

doi:10.1016/j.mimet.2004.11.018

Removal of contaminating DNA from commercial nucleic acid extraction kit reagents

J Microbiol Methods. 2005 May;61(2):285-8. doi: 10.1016/j.mimet.2004.11.018. Epub 2004 Dec 15.

Authors

Tamimount Mohammadi¹, Henk W Reesink, Christina M J E Vandenbroucke-Grauls, Paul H M Savelkoul

Affiliation

¹ Sanquin Blood Bank North West Region, Amsterdam, The Netherlands.

PMID: 15722157
DOI: 10.1016/j.mimet.2004.11.018

Abstract

Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Platelets / microbiology
DNA, Bacterial / isolation & purification*
False Positive Reactions
Humans
RNA, Ribosomal, 16S / analysis
Reagent Kits, Diagnostic / standards*

Substances

DNA, Bacterial
RNA, Ribosomal, 16S
Reagent Kits, Diagnostic