Leucine aminopeptidase M inhibitors, cyanostatin A and B, isolated from cyanobacterial water blooms in Scotland

Tomoharu Sano; Hiroo Takagi; Louise F Morrison; James S Metcalf; Geoffrey A Codd; Kunimitsu Kaya

doi:10.1016/j.phytochem.2005.01.001

Leucine aminopeptidase M inhibitors, cyanostatin A and B, isolated from cyanobacterial water blooms in Scotland

Phytochemistry. 2005 Mar;66(5):543-8. doi: 10.1016/j.phytochem.2005.01.001.

Authors

Tomoharu Sano¹, Hiroo Takagi, Louise F Morrison, James S Metcalf, Geoffrey A Codd, Kunimitsu Kaya

Affiliation

¹ National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. sanotomo@nies.go.jp

PMID: 15721946
DOI: 10.1016/j.phytochem.2005.01.001

Abstract

Two leucine aminopeptidase M inhibitors, cyanostatin A and B, were isolated from cyanobacterial water blooms at Loch Rescobie in Scotland, and specifically from a Microcystis species. Both inhibitors were lipopeptides containing 3-amino-2-hydroxydecanoic acid and weak inhibitors of protein phosphatase (PP2A). Both strongly inhibited the activity of leucine aminopeptidase M with IC50 values of 40 and 12 ng/ml, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anabaena
Cyanobacteria / isolation & purification*
Enzyme Inhibitors / isolation & purification*
Enzyme Inhibitors / pharmacology
Kinetics
Leucyl Aminopeptidase / antagonists & inhibitors*
Microcystis
Oligopeptides / isolation & purification
Oligopeptides / pharmacology*
Water Microbiology*

Substances

Enzyme Inhibitors
Oligopeptides
cyanostatin A
cyanostatin B
Leucyl Aminopeptidase