The promoter region rather than its downstream inverted repeat sequence is responsible for low-level transcription of the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus

Yoshitsugu Nakaguchi; Mitsuaki Nishibuchi

doi:10.1128/JB.187.5.1849-1855.2005

The promoter region rather than its downstream inverted repeat sequence is responsible for low-level transcription of the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus

J Bacteriol. 2005 Mar;187(5):1849-55. doi: 10.1128/JB.187.5.1849-1855.2005.

Authors

Yoshitsugu Nakaguchi¹, Mitsuaki Nishibuchi

Affiliation

¹ Graduate School of Medicine, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.

Abstract

We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / biosynthesis
Bacterial Proteins / genetics*
Base Sequence
Chromosome Mapping
Gene Expression Regulation, Bacterial / physiology
Hemolysin Proteins / biosynthesis
Hemolysin Proteins / genetics*
Molecular Sequence Data
Mutation
Promoter Regions, Genetic / physiology*
Recombinant Fusion Proteins
Repetitive Sequences, Nucleic Acid / genetics
Repetitive Sequences, Nucleic Acid / physiology*
Sequence Alignment
Transcription, Genetic / physiology*
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / metabolism*

Substances

Bacterial Proteins
Hemolysin Proteins
Recombinant Fusion Proteins
thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus

Associated data

GENBANK/AB112353
GENBANK/AB112354
GENBANK/AB112355