Abstract
Following antigen recognition, murine CD8 T cells express CD94/NKG2A receptors. Our results show that this up-regulation occurs rapidly in vitro and is accompanied by an approximately 8-fold increase in CD94 and approximately 125-fold increase in NKG2A mRNA. In contrast, only a twofold increase in NKG2C mRNA is noted. The addition of TGF-beta, but not IL-10, IL-12 or IL-15, leads to a further increase in cell membrane expression of these receptors, as well as a approximately 6-fold increase in mRNA for both chains. TGF-beta also increases CD94/NKG2A expression on memory CD8 T cells that are re-exposed to antigen. The effect of TGF-beta on increasing CD94/NKG2A expression on both naive and memory CD8 T cells occurs only when there is a concurrent stimulation through the TCR. In contrast, TGF-beta does not increase expression of CD94/NKG2A on resting or activated NK cells. We also show by using purified CD8 T cells, that TGF-beta acts directly on these cells. These results implicate a role for both antigen and TGF-beta in increasing expression of inhibitory CD94/NKG2A receptors on CD8 T cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD / immunology*
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Antigens, CD / metabolism
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CD8-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / metabolism
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Flow Cytometry
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Gene Expression
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Lectins, C-Type / immunology*
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Lectins, C-Type / metabolism
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Listeria monocytogenes / immunology
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Listeriosis / immunology
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Mice
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NK Cell Lectin-Like Receptor Subfamily C
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NK Cell Lectin-Like Receptor Subfamily D
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RNA, Messenger / analysis
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Receptors, Antigen, T-Cell / immunology*
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Receptors, Antigen, T-Cell / metabolism
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Receptors, Immunologic / immunology*
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Receptors, Immunologic / metabolism
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Receptors, Natural Killer Cell
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Reverse Transcriptase Polymerase Chain Reaction
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Transforming Growth Factor beta / immunology*
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Transforming Growth Factor beta / metabolism
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Up-Regulation
Substances
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Antigens, CD
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Klrc1 protein, mouse
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Klrc2 protein, mouse
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Klrd1 protein, mouse
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Lectins, C-Type
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NK Cell Lectin-Like Receptor Subfamily C
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NK Cell Lectin-Like Receptor Subfamily D
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RNA, Messenger
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Receptors, Antigen, T-Cell
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Receptors, Immunologic
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Receptors, Natural Killer Cell
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Transforming Growth Factor beta