Objective: To find out the optimal extraction technique for HDCA (a-hydroxycholic acid).
Method: According to the orthogonal design L9(3(4)), the optimal extraction technique was sought through experimental investigation, and the content of HDCA was determined by TLC.
Result: The optimal extraction method was eightfold 8% NaOH solution and 16 hours. The optimal purification method was six fold ethyl acetate, 5% active carbon, and 30 minutes twice.
Conclusion: The above mentioned extraction technique is optimal and feasible in extraction of HDCA.