The characterization of lubricity improver additives by GC-MS-SIM is an important tool for the development and monitoring of new formulations as well as for quality control of additives in diesel. A simple method for the quantification of a linoleic oil additive derivative in diesel has been developed. Calibration is performed by analysis of standard solutions containing 2-hydroxy-ethyl linoleate with pentadecanoyl propanoate as the internal standard. The proposed method permitted the quantification of the linoleate ester at a concentration of 300 microg/mL in the additived diesel. The recovery of additive and the repeatability of the quantitative results are evaluated.