Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography

Li Li; Rong Tsao; Zhiqiang Liu; Shuying Liu; Raymond Yang; J Christopher Young; Honghui Zhu; Zeyuan Deng; Mingyong Xie; Zhihong Fu

doi:10.1016/j.chroma.2004.11.024

Isolation and purification of acteoside and isoacteoside from Plantago psyllium L. by high-speed counter-current chromatography

J Chromatogr A. 2005 Jan 21;1063(1-2):161-9. doi: 10.1016/j.chroma.2004.11.024.

Authors

Li Li¹, Rong Tsao, Zhiqiang Liu, Shuying Liu, Raymond Yang, J Christopher Young, Honghui Zhu, Zeyuan Deng, Mingyong Xie, Zhihong Fu

Affiliation

¹ ChangChun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Science, ChangChun, PR China.

PMID: 15700468
DOI: 10.1016/j.chroma.2004.11.024

Abstract

Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.

MeSH terms

Chromatography, High Pressure Liquid
Countercurrent Distribution / methods*
Glucosides / isolation & purification*
Mass Spectrometry
Phenols / isolation & purification*
Plantago / chemistry*
Spectrophotometry, Ultraviolet

Substances

Glucosides
Phenols
acteoside