Abstract
A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Esterases / genetics
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Esterases / isolation & purification
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Esterases / metabolism
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Histidine / chemistry
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Protein Array Analysis / methods*
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification*
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Recombinant Proteins / metabolism*
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Surface Plasmon Resonance / methods*
Substances
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Recombinant Proteins
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Histidine
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Esterases